|Recombinant Protein Services|
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In recent decade, genomic and proteomic tools has enabled to identify genes and proteins potentially involved in different biological process.
We can isolate for you the gene of interest either by RT-PCR or PCR from provided mRNA, cDNA or DNA. We also have the gene synthesis service for codon optimization, mutagenesis, etc. This step is validated by sequencing.
We clone routinely into a wide range of vectors for expression in E. coli, Yeast, and insect cells carrying different tags and regulatory sequences. For special requirement, vectors can be customized according to your needs (Tag, promoter, …). Each construction is validated by sequencing and reported on.
In all cases, for functional and biochemical characterization (enzymatic activity, binding…) and structural studies we provide amounts of protein ranging from µg to grams.
Once the production and purification parameters are optimized, or transferred when available elsewhere, we go to the final batch production:
This step is crucial for the success of the project. Our expertise and know-how associated with suitable facilities guarantee purification in the best conditions, saving thereby the integrity and properties of proteins.
Basically, the purification is based on affinity chromatography. However, if a high purity grade or polishing step is required, we combine/associate additional techniques: ions exchange, gel filtration, hydrophobic interaction chromatography. We also purify protein without tags.
You can entrust us your production projects off or regular:
The proteins are expressed in different prokaryotic systems (E. coli) and/or eukaryotic (yeast and insect cell / baculovirus, as cytosolic or secreted proteins).
Depending on your needs we can deliver proteins endotoxin free, and high levels of purity> 97% for structural studies (crystallography, NMR).
It depends on gene size, expression system, protein type and required purity. Here are the required time for each step and expression system.
Initially, we conduct a feasibility study for the project and set up the necessary protocols for protein expression and purification. After validation of the feasibility study (production assay, yield evaluation), a report is sent to the client.
According to customer requirement, proteins preparation are lyophilised or preserved in a specific buffer before sending.
If you would like to have a quotation, please fill out:
For any request, please contact:
|PROTENIA S.A||Al-Akhawayn Technopark||Tel. : +212 (0) 5.35-56-74-03||Ifrane - 53 000||Morocco|
|Conception: G.iDEA||production, protein, recombinant, extraction, purification, DNA, RNA, cloning, sequencing||Copyright 2008 - 2014 ©|